The present study was aimed to investigate the anticancer property of polysaccharide of Ganoderma lucidum. Mushroom Ganoderma lucidum was collected from the mangrove environment. Hot water extraction and ethanol precipitation method was used for extracting crude polysaccharide. Yield of crude polysaccharide was recorded as 20.5%, total sugar content of the polysaccharides was found to be 95.6% estimated by phenol-sulfuric acid method. Fourier transform infrared spectroscopy was used to identify the functional characteristic absorption of polysaccharide, the IR band around at 781.17 cm-1 indicated the presence of sulfate ester groups, which signifies that it was a sulfated polysaccharide. The crude hot water polysaccharide was partially purified by DEAE-cellulose column (16.5 × 1.3 cm) column chromatography. A total of 28 fractions of each 5 ml were collected among these three active fractions (F-17, F-18 and F-19) were subjected for anticancer activity against cervical cancer cell lines (HeLa) in different concentrations using MTT assay. In this study, Fraction 19 showed highest cytotoxicity against Hela cell line with IC50 values of 271.46 µg/ml. The obtained results in this study showed that the sulfated polysaccharide have the ability to reduce the risk of cancer through their cytotoxicity activities.
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